3WRY
Crystal structure of helicase complex 2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-17A |
Synchrotron site | Photon Factory |
Beamline | BL-17A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-12-06 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 84.981, 133.550, 195.786 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.750 - 2.300 |
R-factor | 0.202 |
Rwork | 0.202 |
R-free | 0.24800 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 1.200 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | CNS (1.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 33.750 | 2.340 |
High resolution limit [Å] | 2.300 | 2.300 |
Number of reflections | 99309 | |
Completeness [%] | 99.6 | 98.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7.5 | 293 | 0.2M L-proline, 0.1M HEPES-KOH, pH7.0, 11%(w/v) PEG 3350, pH 7.5, VAPOR DIFFUSION, temperature 293K |