3WRU
Crystal structure of the bacterial ribosomal decoding site in complex with synthetic aminoglycoside with F-HABA group
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-10-27 |
Detector | ADSC QUANTUM 315r |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 46.554, 32.602, 51.413 |
Unit cell angles | 90.00, 108.50, 90.00 |
Refinement procedure
Resolution | 39.700 - 2.300 |
Rwork | 0.249 |
R-free | 0.26200 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.005 |
RMSD bond angle | 0.921 |
Data reduction software | CrystalClear |
Data scaling software | CrystalClear |
Phasing software | PHENIX |
Refinement software | CNS |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 39.700 |
High resolution limit [Å] | 2.300 |
Number of reflections | 6668 |
Redundancy | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | Na Cacodylate, Spermine, KCl, MPD, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |