3WR5
Structural basis on the efficient CO2 reduction of acidophilic formate dehydrogenase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B1 |
Synchrotron site | SPring-8 |
Beamline | BL26B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-01-19 |
Detector | RIGAKU SATURN A200 |
Wavelength(s) | 0.8000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 55.942, 72.729, 386.767 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.250 - 2.142 |
R-factor | 0.1742 |
Rwork | 0.172 |
R-free | 0.20850 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2nad |
RMSD bond length | 0.009 |
RMSD bond angle | 1.355 |
Data reduction software | DENZO |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.180 |
High resolution limit [Å] | 2.140 | 2.140 |
Number of reflections | 88253 | |
Completeness [%] | 99.9 | 100 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 1.4M Lithium chloride, 0.1M Tris-HCl pH 7.5, 24% PEG 6000, VAPOR DIFFUSION, SITTING DROP, temperature 298K |