3WQP
Crystal structure of Rubisco T289D mutant from Thermococcus kodakarensis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 170.566, 246.238, 144.628 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.250 |
| R-factor | 0.222 |
| Rwork | 0.220 |
| R-free | 0.25830 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 3a12 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.006 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.290 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.404 | |
| Number of reflections | 287149 | |
| <I/σ(I)> | 13.1 | 3.3 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 7.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6 | 293 | Sodium Acetate 100mM (pH6.0), PEG 6000 5%(w/v), 2-methyl-1,4-pentanediol 10%(v/v), Calcium Chloride 120mM, VAPOR DIFFUSION, temperature 293K |
