3WQ8
Monomer structure of hyperthermophilic beta-glucosidase mutant forming a dodecameric structure in the crystal form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-01-01 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.90000 |
| Spacegroup name | P 1 |
| Unit cell lengths | 97.365, 148.872, 148.563 |
| Unit cell angles | 120.08, 94.00, 99.70 |
Refinement procedure
| Resolution | 48.280 - 2.810 |
| R-factor | 0.2446 |
| Rwork | 0.243 |
| R-free | 0.27497 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3wdp |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.480 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 48.280 |
| High resolution limit [Å] | 2.810 |
| Rmerge | 0.110 |
| Number of reflections | 170856 |
| <I/σ(I)> | 28.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.6 | 293 | 0.1M HEPES-NaOH pH7.6, 0.2M Ca-Acetate, 20% PEG 3350, 5% Ethyleneglycol, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
