3WPX
Structure of PomBc4, a periplasmic fragment of PomB from Vibrio alginolyticus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL32XU |
Synchrotron site | SPring-8 |
Beamline | BL32XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-02-16 |
Detector | RAYONIX MX225HE |
Wavelength(s) | 0.97910 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 58.408, 63.300, 77.213 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 32.960 - 2.300 |
R-factor | 0.1812 |
Rwork | 0.175 |
R-free | 0.23410 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3wpw |
RMSD bond length | 0.006 |
RMSD bond angle | 1.010 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHENIX |
Refinement software | PHENIX ((phenix.refine: 1.7.1_743)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.600 | 2.420 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.099 | 0.391 |
Number of reflections | 12786 | |
<I/σ(I)> | 9.7 | 3.7 |
Completeness [%] | 97.2 | 98.5 |
Redundancy | 4.6 | 4.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 20%(w/v) PEGMME2000, 0.1M imidazole, 4%(v/v) MPD, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |