3WPQ

crystal structure of the GAP domain of MgcRacGAP(S387A)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E SUPERBRIGHT
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2013-04-24
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 1 21 1
Unit cell lengths	48.960, 53.610, 86.388
Unit cell angles	90.00, 97.03, 90.00

Refinement procedure

Resolution	28.890 - 1.840
R-factor	0.167
Rwork	0.167
R-free	0.20000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2ovj
RMSD bond length	0.005
RMSD bond angle	1.200
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.910
High resolution limit [Å]	1.840	1.840
Number of reflections	38896
<I/σ(I)>	17.1	3.1
Completeness [%]	100.0	100
Redundancy	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	1.0M Na Citrate, 0.1M Hepes, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K