3WPF

Crystal structure of mouse TLR9 (unliganded form)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	100
Detector technology	CCD
Collection date	2012-10-21
Detector	ADSC QUANTUM 270
Wavelength(s)	0.9800
Spacegroup name	P 21 21 21
Unit cell lengths	71.260, 119.280, 130.410
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.614 - 1.959
R-factor	0.2275
Rwork	0.226
R-free	0.26500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3w3j
RMSD bond length	0.006
RMSD bond angle	1.170
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	PHENIX (1.8.1_1168)

Data quality characteristics

	Overall
Low resolution limit [Å]	48.110
High resolution limit [Å]	1.959
Number of reflections	79163

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	293	6-8% PEG 8000, 0.4M ammonium sulfate, 0.1M Tris pH 8.0, 10% DMSO , VAPOR DIFFUSION, SITTING DROP, temperature 293K