3WPB
Crystal structure of horse TLR9 (unliganded form)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-04-21 |
Detector | RAYONIX MX225HE |
Wavelength(s) | 1.0000 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 98.100, 118.840, 77.750 |
Unit cell angles | 90.00, 108.70, 90.00 |
Refinement procedure
Resolution | 36.850 - 2.400 |
R-factor | 0.20008 |
Rwork | 0.197 |
R-free | 0.25031 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 1.250 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 36.850 |
High resolution limit [Å] | 2.400 |
Number of reflections | 31314 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 293 | 14-10% PEG 3350, 0.2 M ammonium sulfate, 0.1 M Na acetate pH 4.5, 10% ethylene glycol , VAPOR DIFFUSION, SITTING DROP, temperature 293K |