3WOZ
Crystal structure of CLASP2 TOG domain (TOG3)
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-11-27 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97893, 0.97921, 0.96399 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.896, 122.317, 86.514 |
| Unit cell angles | 90.00, 94.67, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.200 |
| R-factor | 0.24252 |
| Rwork | 0.241 |
| R-free | 0.27941 |
| Structure solution method | MAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.898 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.220 |
| High resolution limit [Å] | 2.200 | 5.700 | 2.180 |
| Number of reflections | 56897 | ||
| Completeness [%] | 99.5 | 97.5 | 98.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICRODIALYSIS | 8 | 277 | 10mM Tris, 50mM NaCl, 2mM DTT, pH 8.0, MICRODIALYSIS, temperature 277K |
