3WOR
Crystal structure of the DAP BII octapeptide complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-1A |
Synchrotron site | Photon Factory |
Beamline | BL-1A |
Temperature [K] | 95 |
Detector technology | PIXEL |
Collection date | 2012-06-23 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 1.10000 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 122.008, 122.008, 219.157 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.100 |
R-factor | 0.16896 |
Rwork | 0.166 |
R-free | 0.22642 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3woi |
RMSD bond length | 0.018 |
RMSD bond angle | 1.807 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 30.000 |
High resolution limit [Å] | 2.100 |
Number of reflections | 91253 |
Completeness [%] | 100.0 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9.5 | 293 | 18% PEG 8000, 20% Glycerol, 2mM ZnCl2, 80mM CHES, 2mM Angiotensin II, pH 9.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |