Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 56.425, 56.425, 122.838 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 51.274 - 2.500 |
| R-factor | 0.22 |
| Rwork | 0.217 |
| R-free | 0.27070 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2uvd |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.142 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 51.274 |
| High resolution limit [Å] | 2.500 |
| Number of reflections | 7354 |
| Completeness [%] | 75.0 |
| Redundancy | 9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 277 | 1.5M Ammonium sulfate, 0.1M Tris, 12%(v/v) Glycerol, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
