3WOF
Crystal structure of P23-45 gp39 (6-132) bound to Thermus thermophilus RNA polymerase beta-flap domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NE3A |
Synchrotron site | Photon Factory |
Beamline | AR-NE3A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-02-11 |
Detector | ADSC QUANTUM 270 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 213.777, 213.777, 234.598 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 34.549 - 3.298 |
R-factor | 0.2205 |
Rwork | 0.219 |
R-free | 0.24640 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3woe |
RMSD bond length | 0.009 |
RMSD bond angle | 1.227 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 3.298 |
Number of reflections | 82014 |
<I/σ(I)> | 14.2 |
Completeness [%] | 99.9 |
Redundancy | 10.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 5.6 | 293 | 50mM MES-NaOH (pH 5.6), 6% PEG 8000, 200mM KCl, 10mM MgCl2, VAPOR DIFFUSION, SITTING DROP, temperature 293K |