3WOC
Crystal structure of a prostate-specific WGA16 glycoprotein lectin, form II
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-01-30 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.00000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 134.483, 77.848, 87.187 |
| Unit cell angles | 90.00, 91.30, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.400 |
| R-factor | 0.25569 |
| Rwork | 0.252 |
| R-free | 0.31954 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3aqg |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.015 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 87.040 | 2.440 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.069 | 0.612 |
| Number of reflections | 35861 | |
| <I/σ(I)> | 22.4 | 2.2 |
| Completeness [%] | 99.7 | 99.3 |
| Redundancy | 5.7 | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.1M MES pH 6.5, 1.6M (NH4)2SO4, 10%(v/v) 1,4-dioxane, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
