3WO7
Crystal structure of YidC from Bacillus halodurans (form II)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL32XU |
Synchrotron site | SPring-8 |
Beamline | BL32XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-10-10 |
Detector | RAYONIX MX225HE |
Wavelength(s) | 1.378 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 54.033, 70.058, 82.910 |
Unit cell angles | 90.00, 92.05, 90.00 |
Refinement procedure
Resolution | 45.000 - 3.201 |
R-factor | 0.263 |
Rwork | 0.260 |
R-free | 0.29060 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | SeM-substituted protein structure |
RMSD bond length | 0.002 |
RMSD bond angle | 0.765 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHENIX ((Phaser-MR)) |
Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 45.998 | 3.400 | |
High resolution limit [Å] | 3.200 | 9.430 | 3.200 |
Rmerge | 0.110 | 0.035 | 0.472 |
Rmeas | 0.041 | 0.549 | |
Number of reflections | 19141 | 745 | 3236 |
<I/σ(I)> | 10.13 | 26.44 | 2.96 |
Completeness [%] | 95.6 | 94.9 | 99.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | lipidic qubic phase | 6 | 293 | 24-26% PEG500DME, 10mM CuCl2, 200mM NH4COOH, 100mM MES-NaOH, pH 6.0, lipidic qubic phase, temperature 293K |