3WNK
Crystal Structure of Bacillus circulans T-3040 cycloisomaltooligosaccharide glucanotransferase
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-6A |
Synchrotron site | Photon Factory |
Beamline | BL-6A |
Temperature [K] | 95 |
Detector technology | CCD |
Collection date | 2007-05-23 |
Detector | ADSC QUANTUM 4r |
Wavelength(s) | 0.97800, 0.97885, 0.97931, 0.98319, 0.96408 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 106.437, 106.437, 160.625 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 44.340 - 2.300 |
R-factor | 0.15938 |
Rwork | 0.158 |
R-free | 0.19203 |
Structure solution method | MAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.235 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.340 | 2.380 |
High resolution limit [Å] | 2.300 | 2.300 |
Number of reflections | 47410 | |
<I/σ(I)> | 47.1 | 10.6 |
Completeness [%] | 100.0 | 100 |
Redundancy | 16.6 | 16.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 1.0M sodium acetate trihydrate, 0.1M HEPES, 0.05M cadmium sulfate hydrate, 12%(v/v) glycerol, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |