3WNC

Crystal structure of EF-Pyl in complex with GDP

Experimental procedure

Experimental method	SAD
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	100
Detector technology	CCD
Collection date	2004-10-12
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97943
Spacegroup name	P 21 21 2
Unit cell lengths	62.020, 108.700, 58.390
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.490 - 1.900
R-factor	0.172
Rwork	0.172
R-free	0.21700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3wnb
RMSD bond length	0.009
RMSD bond angle	1.600
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	CNS (1.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.900
Number of reflections	31405
Completeness [%]	99.0
Redundancy	6.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.8	277	Na/Acetate, Na/Citrate, pH 4.8, VAPOR DIFFUSION, HANGING DROP, temperature 277K