3WN1
Crystal Structure of Streptomyces coelicolor alpha-L-arabinofuranosidase in complex with xylotriose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2011-02-14 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 97.668, 97.668, 104.019 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 31.070 - 2.000 |
| R-factor | 0.1802 |
| Rwork | 0.179 |
| R-free | 0.20449 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3wmy |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.510 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | REFMAC (5.7.0029) |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.070 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 34678 | |
| <I/σ(I)> | 42.9 | 13.8 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 17.3 | 17.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.3 | 293 | 20% PEG 3350, 0.2M tri-potassium citrate, pH 8.3, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
