3WMV
The structure of an anti-cancer lectin mytilec with ligand from the mussel Mytilus galloprovincialis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2013-10-28 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.98000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 80.276, 64.215, 74.282 |
| Unit cell angles | 90.00, 121.51, 90.00 |
Refinement procedure
| Resolution | 28.650 - 1.050 |
| R-factor | 0.13688 |
| Rwork | 0.136 |
| R-free | 0.15531 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.025 |
| RMSD bond angle | 2.186 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.070 |
| High resolution limit [Å] | 1.050 | 1.050 |
| Rmerge | 0.066 | 0.414 |
| Number of reflections | 147442 | |
| Completeness [%] | 98.6 | 97.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | Potassiumu thiocyanate, Bis-tris propane, PEG3350, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
