3WMM
Crystal structure of the LH1-RC complex from Thermochromatium tepidum in C2 form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-01-25 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 0.9 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 227.341, 148.238, 161.792 |
| Unit cell angles | 90.00, 117.59, 90.00 |
Refinement procedure
| Resolution | 47.991 - 3.008 |
| R-factor | 0.3151 |
| Rwork | 0.314 |
| R-free | 0.33740 |
| Structure solution method | MIR |
| RMSD bond length | 0.029 |
| RMSD bond angle | 2.056 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.110 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Number of reflections | 78312 | |
| <I/σ(I)> | 16.2 | 2 |
| Completeness [%] | 82.3 | 65.5 |
| Redundancy | 3.6 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 293 | 20mM Tris-HCl (pH 7.4), 50mM CaCl2, 0.54% DPC, 2% glycerol, 14% PEG 3000, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
