3WMJ
Crystal structure of EIAV vaccine gp45
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 200 |
Detector technology | CCD |
Collection date | 2011-05-09 |
Detector | ENRAF-NONIUS |
Wavelength(s) | 0.9794 |
Spacegroup name | P 63 |
Unit cell lengths | 46.947, 46.947, 101.605 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 26.022 - 1.998 |
R-factor | 0.1886 |
Rwork | 0.187 |
R-free | 0.22470 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 0.867 |
Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 26.022 | 2.020 |
High resolution limit [Å] | 1.998 | 1.998 |
Number of reflections | 8643 | |
Completeness [%] | 11.0 | 95 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 4.6 | 293 | 0.2M NaCl, 0.1M sodium acetate trihydrate, 30%(v/v) (+/-)-2-methyl-2,4-pentanediol, pH 4.6, EVAPORATION, temperature 293K |