3WMF

Crystal structure of an inward-facing eukaryotic ABC multitrug transporter G277V/A278V/A279V mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	90
Detector technology	CCD
Collection date	2011-07-24
Detector	RAYONIX MX225HE
Wavelength(s)	1.0000
Spacegroup name	H 3 2
Unit cell lengths	180.534, 180.534, 156.097
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	34.916 - 2.600
R-factor	0.2093
Rwork	0.207
R-free	0.24570
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.008
RMSD bond angle	1.122
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHENIX (1.7.3_928)
Refinement software	PHENIX (1.7.3_928)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.640
High resolution limit [Å]	2.600	7.050	2.600
Rmerge	0.072	0.030	0.513
Number of reflections	30071
<I/σ(I)>	13.6
Completeness [%]	99.8	98.4	98.2
Redundancy	17	18.8	10.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.3	293	15% (w/v) PEG 2000 MME, 100mM magnesium nitrate, pH 6.3, VAPOR DIFFUSION, SITTING DROP, temperature 293K