3WLR
Crystal Structure Analysis of Plant Exohydrolase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
Synchrotron site | Australian Synchrotron |
Beamline | MX1 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC QUANTUM 210r |
Wavelength(s) | 0.9615 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 100.688, 100.688, 183.294 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.370 - 2.210 |
R-factor | 0.17179 |
Rwork | 0.169 |
R-free | 0.21841 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ieq |
RMSD bond length | 0.020 |
RMSD bond angle | 1.716 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.370 | 2.260 |
High resolution limit [Å] | 2.210 | 2.210 |
Rmerge | 0.990 | |
Number of reflections | 45206 | |
<I/σ(I)> | 2.4 | |
Completeness [%] | 98.8 | 88.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | 75mM HEPES-NaOH pH7.0, 1.2% PEG 400, 1.7M ammonium sulphate , VAPOR DIFFUSION, HANGING DROP, temperature 277K |