3WLH
Crystal Structure Analysis of Plant Exohydrolase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-11-12 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 101.015, 101.015, 182.060 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 31.940 - 1.650 |
| R-factor | 0.16244 |
| Rwork | 0.161 |
| R-free | 0.19294 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ieq |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.583 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.3.0040) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 31.940 |
| High resolution limit [Å] | 1.650 |
| Rmerge | 0.089 |
| Number of reflections | 106290 |
| <I/σ(I)> | 1 |
| Completeness [%] | 98.5 |
| Redundancy | 13 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | 75mM HEPES-NaOH pH7.0 buffer, 1.2% PEG 400, 1.7M ammonium sulphate, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
