3WKZ
Crystal Structure of the Ostrinia furnacalis Group I Chitinase catalytic domain E148Q mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-10-03 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0 |
Spacegroup name | P 65 |
Unit cell lengths | 94.241, 94.241, 122.195 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 38.706 - 2.001 |
R-factor | 0.1598 |
Rwork | 0.159 |
R-free | 0.18560 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3w4r |
RMSD bond length | 0.009 |
RMSD bond angle | 1.170 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.030 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.102 | 0.367 |
Number of reflections | 41444 | |
<I/σ(I)> | 22.17 | 10.77 |
Completeness [%] | 100.0 | 100 |
Redundancy | 17 | 17 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | 100mM HEPES pH 8.0, 21% (w/v) PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 277K |