3WKV
Voltage-gated proton channel: VSOP/Hv1 chimeric channel
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-02-05 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 0.97884, 0.97930, 0.96407 |
| Spacegroup name | P 63 |
| Unit cell lengths | 86.540, 86.540, 89.553 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.327 - 3.453 |
| R-factor | 0.3421 |
| Rwork | 0.341 |
| R-free | 0.35700 |
| Structure solution method | MAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.500 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELX |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.510 |
| High resolution limit [Å] | 3.450 | 9.350 | 3.450 |
| Rmerge | 0.042 | 0.221 | 0.747 |
| Number of reflections | 5035 | ||
| <I/σ(I)> | 44.377 | 53.9 | 1.98 |
| Completeness [%] | 99.1 | 83.6 | 100 |
| Redundancy | 5.6 | 6 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8 | 293 | PEG400, KCl, pH 8.0, vapor diffusion, temperature 293K |
