3WIJ
Crystal structure of a plant class V chitinase mutant from Cycas revoluta in complex with (GlcNAc)3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2011-02-21 |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 57.651, 63.916, 86.048 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.690 - 1.300 |
| R-factor | 0.18023 |
| Rwork | 0.179 |
| R-free | 0.19712 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3alg |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.486 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.320 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Number of reflections | 78915 | |
| Completeness [%] | 100.0 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 60%(v/v) Tacsimate, 0.1M BIS-TRIS propane, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
