3WIA
Crystal structure of the N-terminal 1-37 residues deleted mutant of Geobacillus copper nitrite reductase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-06-24 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 89.317, 164.991, 126.561 |
| Unit cell angles | 90.00, 102.27, 90.00 |
Refinement procedure
| Resolution | 42.700 - 1.770 |
| R-factor | 0.1723 |
| Rwork | 0.171 |
| R-free | 0.19243 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3wi9 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.435 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 124.000 | 1.800 |
| High resolution limit [Å] | 1.770 | 1.770 |
| Number of reflections | 346257 | |
| Completeness [%] | 98.6 | 98.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 289 | 0.1M Hepes-NaOH, 2.0M Ammonium formate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
