3WI5
Crystal structure of the Loop 7 mutant PorB from Neisseria meningitidis serogroup B
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-11-22 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1.00 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 143.486, 143.486, 178.420 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 2.400 |
| R-factor | 0.22946 |
| Rwork | 0.227 |
| R-free | 0.25596 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3vzt |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.638 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MLPHARE |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.450 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Number of reflections | 25466 | |
| Completeness [%] | 92.2 | 73 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 65mM HEPES pH 7.5, 1.1M tri-sodium citrate, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
