3WI1
P453H/I471T mutant of PB2 middle domain from influenza virus A/Puerto Rico/8/34(H1N1) with m7GTP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2013-08-05 |
Detector | RIGAKU RAXIS VII |
Wavelength(s) | 1.54 |
Spacegroup name | H 3 2 |
Unit cell lengths | 107.672, 107.672, 138.302 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 18.891 - 1.931 |
R-factor | 0.1784 |
Rwork | 0.175 |
R-free | 0.21240 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2vqz |
RMSD bond length | 0.006 |
RMSD bond angle | 1.076 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.280 | 1.820 |
High resolution limit [Å] | 1.760 | 1.760 |
Number of reflections | 30518 | |
Completeness [%] | 99.9 | 99.73 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | 1.2M NaCl, 2.5% (v/v) ethanol, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |