3WHJ
Crystal structure of Nas2 N-terminal domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Detector technology | CCD |
| Collection date | 2012-08-04 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97946 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 64.590, 64.590, 149.760 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 1.650 |
| R-factor | 0.161 |
| Rwork | 0.159 |
| R-free | 0.19700 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.571 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.680 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.088 | 0.391 |
| Number of reflections | 23180 | |
| <I/σ(I)> | 45.7 | 4.4 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 10 | 10.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 0.9M sodium acetate trihydrate, 0.1M HEPES (pH 8.5), 50mM cadmium sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
