3WHE
A new conserved neutralizing epitope at the globular head of hemagglutinin in H3N2 influenza viruses
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-10-22 |
Detector | RAYONIX MX225HE |
Wavelength(s) | 1.00 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 391.037, 241.173, 223.214 |
Unit cell angles | 90.00, 123.62, 90.00 |
Refinement procedure
Resolution | 30.000 - 4.000 |
R-factor | 0.236 |
Rwork | 0.235 |
R-free | 0.30840 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ha0 |
RMSD bond length | 0.004 |
RMSD bond angle | 1.023 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASES |
Refinement software | CNS (1.3) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 30.000 | 30.000 | 4.250 |
High resolution limit [Å] | 4.000 | 20.000 | 4.000 |
Rmerge | 0.236 | 2.100 | 2.100 |
Rmeas | 0.025 | 2.433 | |
Number of reflections | 142111 | 817 | 23528 |
<I/σ(I)> | 6.35 | 48.96 | 0.66 |
Completeness [%] | 98.0 | 66.4 | 98.1 |
Redundancy | 3.905 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | PEG8000, KCl, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |