3WHE
A new conserved neutralizing epitope at the globular head of hemagglutinin in H3N2 influenza viruses
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-10-22 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 1.00 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 391.037, 241.173, 223.214 |
| Unit cell angles | 90.00, 123.62, 90.00 |
Refinement procedure
| Resolution | 30.000 - 4.000 |
| R-factor | 0.236 |
| Rwork | 0.235 |
| R-free | 0.30840 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ha0 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.023 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASES |
| Refinement software | CNS (1.3) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 4.250 |
| High resolution limit [Å] | 4.000 | 20.000 | 4.000 |
| Rmerge | 0.236 | 2.100 | 2.100 |
| Rmeas | 0.025 | 2.433 | |
| Number of reflections | 142111 | 817 | 23528 |
| <I/σ(I)> | 6.35 | 48.96 | 0.66 |
| Completeness [%] | 98.0 | 66.4 | 98.1 |
| Redundancy | 3.905 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | PEG8000, KCl, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
