3WH3
human Mincle, ligand free form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-10-22 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0 |
Spacegroup name | P 31 |
Unit cell lengths | 49.074, 49.074, 43.042 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 24.537 - 1.320 |
R-factor | 0.1391 |
Rwork | 0.137 |
R-free | 0.17110 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1fm5 |
RMSD bond length | 0.006 |
RMSD bond angle | 1.133 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 24.540 | 1.367 |
High resolution limit [Å] | 1.320 | 1.320 |
Rmerge | 0.053 | 0.419 |
Number of reflections | 27209 | |
<I/σ(I)> | 17.7 | 3.3 |
Completeness [%] | 100.0 | 98.53 |
Redundancy | 5.3 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.1M Bis-tris propane, 0.2M potassium thiocyanate, 20%(w/v) PEG3350, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |