3WGT
Crystal structure of D-amino acid oxidase mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-17A |
Synchrotron site | Photon Factory |
Beamline | BL-17A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-05-29 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.98 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 68.617, 91.607, 110.319 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 34.130 - 1.880 |
R-factor | 0.19054 |
Rwork | 0.188 |
R-free | 0.23031 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1AA8 |
RMSD bond length | 0.011 |
RMSD bond angle | 1.672 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 34.130 | 1.910 |
High resolution limit [Å] | 1.880 | 1.880 |
Number of reflections | 56667 | |
Completeness [%] | 97.7 | 99.8 |
Redundancy | 5.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 30% Polyethylene glycole 4000, 0.1M TRIS hydrochloride, 0.2M Lithium sulfate monohydrate, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |