3WGN

STAPHYLOCOCCUS AUREUS FTSZ bound with GTP-gamma-S

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	100
Detector technology	CCD
Collection date	2011-12-04
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9800
Spacegroup name	P 1
Unit cell lengths	44.270, 44.170, 73.280
Unit cell angles	82.84, 82.81, 82.34

Refinement procedure

Resolution	43.511 - 2.606
R-factor	0.2306
Rwork	0.228
R-free	0.27990
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3voa
RMSD bond length	0.012
RMSD bond angle	1.591
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX (1.8_1069)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000		2.760
High resolution limit [Å]	2.606	7.710	2.610
Rmerge	0.091	0.033	0.610
Rmeas		0.038	0.710
Number of reflections	16267	641	2577
<I/σ(I)>	11.79	32.11	2.43
Completeness [%]	98.4	99.4	96.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	1.0M Lithium chloride, 0.1M Sodium acetate, 30%(w/v) PEG6000, 10%(v/v) glycerol, 5mM GTPgammaS, VAPOR DIFFUSION, SITTING DROP, temperature 293K