3WGM
STAPHYLOCOCCUS AUREUS FTSZ T7 mutant substituted for GAN bound with GTP, DeltaT7GAN-GTP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-04-10 |
Detector | RAYONIX MX225HE |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 44.530, 89.640, 66.580 |
Unit cell angles | 90.00, 96.11, 90.00 |
Refinement procedure
Resolution | 44.820 - 2.091 |
R-factor | 0.1947 |
Rwork | 0.193 |
R-free | 0.22670 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3voa |
RMSD bond length | 0.005 |
RMSD bond angle | 1.023 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | PHENIX (1.8.1_1168) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.220 | |
High resolution limit [Å] | 2.090 | 6.220 | 2.090 |
Rmerge | 0.118 | 0.054 | 0.778 |
Rmeas | 0.063 | 0.905 | |
Number of reflections | 30298 | 1193 | 4729 |
<I/σ(I)> | 8.13 | 21.11 | 1.98 |
Completeness [%] | 98.4 | 98.8 | 95.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1M Sodium HEPES, 27.5%(w/v) PEG2000MME, vapor diffusion, sitting drop, temperature 293K |