3WGM

STAPHYLOCOCCUS AUREUS FTSZ T7 mutant substituted for GAN bound with GTP, DeltaT7GAN-GTP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	CCD
Collection date	2013-04-10
Detector	RAYONIX MX225HE
Wavelength(s)	1.0
Spacegroup name	P 1 21 1
Unit cell lengths	44.530, 89.640, 66.580
Unit cell angles	90.00, 96.11, 90.00

Refinement procedure

Resolution	44.820 - 2.091
R-factor	0.1947
Rwork	0.193
R-free	0.22670
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3voa
RMSD bond length	0.005
RMSD bond angle	1.023
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX (1.8.1_1168)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000		2.220
High resolution limit [Å]	2.090	6.220	2.090
Rmerge	0.118	0.054	0.778
Rmeas		0.063	0.905
Number of reflections	30298	1193	4729
<I/σ(I)>	8.13	21.11	1.98
Completeness [%]	98.4	98.8	95.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1M Sodium HEPES, 27.5%(w/v) PEG2000MME, vapor diffusion, sitting drop, temperature 293K