3WGL
STAPHYLOCOCCUS AUREUS FTSZ T7 mutant substituted for GAN bound with GDP, DeltaT7GAN-GDP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-1A |
| Synchrotron site | Photon Factory |
| Beamline | BL-1A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-12-16 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1.1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.886, 97.156, 72.559 |
| Unit cell angles | 90.00, 102.41, 90.00 |
Refinement procedure
| Resolution | 48.578 - 3.066 |
| R-factor | 0.2117 |
| Rwork | 0.209 |
| R-free | 0.26500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3wgk |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.911 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.250 | |
| High resolution limit [Å] | 3.066 | 9.050 | 3.070 |
| Rmerge | 0.151 | 0.036 | 0.755 |
| Rmeas | 0.043 | 0.894 | |
| Number of reflections | 11397 | 454 | 1813 |
| <I/σ(I)> | 10.22 | 30.21 | 1.9 |
| Completeness [%] | 99.0 | 98.1 | 97.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1M Sodium HEPES, 30%(w/v) PEG2000MME, vapor diffusion, sitting drop, temperature 293K |
