3WG3
Crystal structure of Agrocybe cylindracea galectin with blood type A antigen tetraose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NE3A |
Synchrotron site | Photon Factory |
Beamline | AR-NE3A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-11-24 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 1.0000 |
Spacegroup name | P 65 |
Unit cell lengths | 102.921, 102.921, 74.694 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 30.230 - 1.350 |
R-factor | 0.14076 |
Rwork | 0.140 |
R-free | 0.15586 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ww7 |
RMSD bond length | 0.006 |
RMSD bond angle | 1.178 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 35.000 | 35.000 | 1.400 |
High resolution limit [Å] | 1.350 | 2.910 | 1.350 |
Rmerge | 0.058 | 0.049 | 0.343 |
Number of reflections | 98551 | ||
<I/σ(I)> | 14.8 | ||
Completeness [%] | 99.9 | 99.5 | 99.9 |
Redundancy | 10.8 | 11 | 8.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 30% PEG 1500, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |