3WFW
Crystal Structure of the Closed Form of the HGbRL's Globin Domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2011-08-23 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.5418 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 67.240, 54.400, 44.710 |
Unit cell angles | 90.00, 106.17, 90.00 |
Refinement procedure
Resolution | 27.514 - 1.650 |
R-factor | 0.1519 |
Rwork | 0.151 |
R-free | 0.16890 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.201 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | MOLREP |
Refinement software | PHENIX (1.8.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.690 |
High resolution limit [Å] | 1.650 | 1.650 |
Rmerge | 0.025 | 0.061 |
Number of reflections | 38021 | |
<I/σ(I)> | 22.01 | 10.96 |
Completeness [%] | 89.6 | 87.3 |
Redundancy | 2.3 | 2.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 277 | 50% 2-methyl-2,4-pentanediol, 0.1M Tris-HCl, 0.2M (NH4)2HPO4, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K |