3WE7
Crystal Structure of Diacetylchitobiose Deacetylase from Pyrococcus horikoshii
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Wavelength(s) | 0.97895, 0.97926, 0.99498 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 77.372, 77.372, 230.223 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.620 - 1.550 |
| R-factor | 0.17501 |
| Rwork | 0.173 |
| R-free | 0.20733 |
| Structure solution method | MAD |
| RMSD bond length | 0.024 |
| RMSD bond angle | 2.346 |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.550 |
| Number of reflections | 115813 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293 | 0.01M cobaltous chloride hexahydrate, 0.1M sodium acetate trihydrate, 1.0M 1,6 hexanediol, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |
