3WDQ
Crystal structure of beta-mannanase from a symbiotic protist of the termite Reticulitermes speratus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-17A |
Synchrotron site | Photon Factory |
Beamline | BL-17A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-11-25 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 45.503, 61.405, 57.806 |
Unit cell angles | 90.00, 92.74, 90.00 |
Refinement procedure
Resolution | 28.870 - 1.300 |
R-factor | 0.14489 |
Rwork | 0.144 |
R-free | 0.16364 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3cbw |
RMSD bond length | 0.011 |
RMSD bond angle | 1.477 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | BALBES |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.320 |
High resolution limit [Å] | 1.300 | 1.300 |
Number of reflections | 77766 | |
<I/σ(I)> | 34.6 | 2.3 |
Completeness [%] | 99.9 | 99.7 |
Redundancy | 7.3 | 6.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | 25% PEG3350, 0.2M magnesium chloride, 0.1M Bis-Tris, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |