3WDQ
Crystal structure of beta-mannanase from a symbiotic protist of the termite Reticulitermes speratus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-11-25 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 45.503, 61.405, 57.806 |
| Unit cell angles | 90.00, 92.74, 90.00 |
Refinement procedure
| Resolution | 28.870 - 1.300 |
| R-factor | 0.14489 |
| Rwork | 0.144 |
| R-free | 0.16364 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3cbw |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.477 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | BALBES |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.320 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Number of reflections | 77766 | |
| <I/σ(I)> | 34.6 | 2.3 |
| Completeness [%] | 99.9 | 99.7 |
| Redundancy | 7.3 | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | 25% PEG3350, 0.2M magnesium chloride, 0.1M Bis-Tris, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
