3WDN
High-resolution X-ray crystal structure of bovine H-protein using a high-pressure cryocooling method
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 2011-04-24 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 0.7 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 84.870, 41.415, 43.317 |
| Unit cell angles | 90.00, 91.72, 90.00 |
Refinement procedure
| Resolution | 30.700 - 0.860 |
| Rwork | 0.114 |
| R-free | 0.13900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3klr |
| RMSD bond length | 0.017 |
| RMSD bond angle | 2.206 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.700 | 0.870 |
| High resolution limit [Å] | 0.860 | 0.860 |
| Rmerge | 0.059 | 0.496 |
| Number of reflections | 126382 | |
| <I/σ(I)> | 44.7 | 2.85 |
| Completeness [%] | 99.8 | 99.3 |
| Redundancy | 5.9 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 3 | 288 | 0.6-1.6M ammonium sulphate, 0-30% glycerol, 0.1M sodium citrate, pH 3.0, VAPOR DIFFUSION, HANGING DROP, temperature 288K |
