3WCR

Crystal structure of plant lectin (ligand-free form)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	95
Detector technology	CCD
Collection date	2012-02-24
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.91939
Spacegroup name	P 41 21 2
Unit cell lengths	68.856, 68.856, 242.539
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	66.240 - 2.450
R-factor	0.21525
Rwork	0.213
R-free	0.25681
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	1.310
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	REFMAC (5.6.0117)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	2.490
High resolution limit [Å]	2.450	2.450
Number of reflections	24455
<I/σ(I)>	26.3	5.2
Completeness [%]	100.0	100
Redundancy	9.8	9.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	3.2	293	0.1M citric acid (pH 3.2), 20% PEG 6000, VAPOR DIFFUSION, SITTING DROP, temperature 293K