3WCQ
Crystal structure analysis of Cyanidioschyzon melorae ferredoxin D58N mutant
Experimental procedure
| Experimental method | SAD |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 34.026, 46.527, 66.139 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 26.970 - 0.970 |
| R-factor | 0.1517 |
| Rwork | 0.151 |
| R-free | 0.17003 |
| Structure solution method | SAD |
| RMSD bond length | 0.025 |
| RMSD bond angle | 2.445 |
| Data reduction software | CrystalClear |
| Data scaling software | CrystalClear |
| Phasing software | CRANK |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 0.970 |
| Rmerge | 0.604 |
| Number of reflections | 57345 |
| Completeness [%] | 96.1 |
| Redundancy | 12.53 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 1.5M ammonium sulfate, pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
