3WCK
Crystal structure of monomeric photosensitizing fluorescent protein, Supernova
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
Synchrotron site | Photon Factory |
Beamline | AR-NW12A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-10-10 |
Detector | ADSC QUANTUM 210 |
Spacegroup name | P 1 |
Unit cell lengths | 60.252, 89.304, 109.885 |
Unit cell angles | 70.57, 89.95, 71.62 |
Refinement procedure
Resolution | 20.000 - 2.300 |
R-factor | 0.184 |
Rwork | 0.181 |
R-free | 0.23400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3a8s |
RMSD bond length | 0.019 |
RMSD bond angle | 1.942 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.340 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.070 | 0.323 |
Number of reflections | 88149 | |
<I/σ(I)> | 23.5 | 5.1 |
Completeness [%] | 97.9 | 97.1 |
Redundancy | 3.1 | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.2 | 293 | 0.1M SODIUM ACETATE, 2.2M SODIUM CHLORIDE, 0.2M LITHIUM SULFATE, pH 4.2, VAPOR DIFFUSION, SITTING DROP, temperature 293K |