3WC3
Crystal structure of endo-1,4-beta-glucanase from Eisenia fetida
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-04-20 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 136.449, 136.449, 54.974 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.200 - 1.500 |
| R-factor | 0.1482 |
| Rwork | 0.147 |
| R-free | 0.16791 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ksd |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.236 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.000 | 1.550 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.099 | 0.356 |
| Number of reflections | 92837 | |
| <I/σ(I)> | 30.4 | 9 |
| Completeness [%] | 98.8 | 97.1 |
| Redundancy | 8.2 | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 293 | 0.2M sodium chloride, 0.6M sodium citrate, 0.067M Tris-HCl, pH 7.0, VAPOR DIFFUSION, temperature 293K |
