3WBR
Crystal structure of carbohydrate recognition domain of Blood Dendritic Cell Antigen-2 (BDCA2) lectin (crystal form-3)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2011-11-07 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 86.130, 86.130, 431.710 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.770 - 2.200 |
| R-factor | 0.25547 |
| Rwork | 0.253 |
| R-free | 0.29479 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2013-May-20.13.55.57.582222.Session.707 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.184 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.330 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Number of reflections | 1204702 | |
| <I/σ(I)> | 21.5 | 6.6 |
| Completeness [%] | 99.9 | 99.3 |
| Redundancy | 7.3 | 14 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.3 | 293 | 0.2M potassium citrate tribasic, 20%(w/v) PEG3350, pH 8.3, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
