3WBJ
Crystal structure analysis of eukaryotic translation initiation factor 5B structure II
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-07-06 |
Detector | RAYONIX MX225HE |
Wavelength(s) | 0.979 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 120.690, 120.690, 168.260 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 44.393 - 2.495 |
R-factor | 0.2175 |
Rwork | 0.215 |
R-free | 0.25580 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3wbi |
RMSD bond length | 0.005 |
RMSD bond angle | 0.938 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 44.393 | 2.650 | |
High resolution limit [Å] | 2.490 | 7.390 | 2.490 |
Rmerge | 0.080 | 0.029 | 0.520 |
Number of reflections | 47589 | ||
<I/σ(I)> | 35.07 | 94.16 | 7.26 |
Completeness [%] | 99.6 | 99 | 98 |
Redundancy | 23.33 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.2 | 293 | 0.1M Tris-HCl pH 8.0, 40% PEG 400, 0.2M Li2SO4, VAPOR DIFFUSION, SITTING DROP, temperature 293K |