3WBI

Crystal structure analysis of eukaryotic translation initiation factor 5B structure I

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	CCD
Collection date	2008-11-21
Detector	ADSC QUANTUM 315
Wavelength(s)	1.000
Spacegroup name	P 41 21 2
Unit cell lengths	129.570, 129.570, 70.510
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.970 - 2.350
R-factor	0.2145
Rwork	0.210
R-free	0.26770
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	1.113
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELXCD
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	43.190		2.490
High resolution limit [Å]	2.350	6.950	2.350
Rmerge	0.091	0.031	0.997
Rmeas		0.032	1.034
Number of reflections	25590	1105	3973
<I/σ(I)>	22.79	60.66	3.19
Completeness [%]	99.6	98.7	97.6
Redundancy	14.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	0.2M Tri-Sodium Citrate, 19% PEG 3350, HEPES pH6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K