3WBH
Structural characteristics of alkaline phosphatase from a moderately halophilic bacteria Halomonas sp.593
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-07-12 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 52.713, 146.974, 58.280 |
| Unit cell angles | 90.00, 105.21, 90.00 |
Refinement procedure
| Resolution | 35.290 - 2.100 |
| R-factor | 0.17931 |
| Rwork | 0.177 |
| R-free | 0.22461 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3e2d |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.576 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 2.140 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.084 | 0.084 |
| Number of reflections | 49534 | |
| <I/σ(I)> | 15.1 | 3.4 |
| Completeness [%] | 98.8 | 99.1 |
| Redundancy | 3.3 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 0.2M MAGNESIUM CHLORIDE HEXAHYDRATE, 0.1M TRIS HYDROCHLORIDE (PH 8.5), 30% PEG 4000, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
